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Abstract:

Medicinal mushrooms are highly valued due to their high levels of vital bioactive constituentswith potential health benefits. Trametes versicolor is a widely known medicinal mushroom utilizedas a nutrient source and for therapeutic purposes. Our study was carried out to examine the nutritionalcontent, phytochemicals, antioxidants and antimicrobial activity of T. versicolor. The mushroom,collected from decaying wood at Phulchowki, Godawari, Lalitpur, was identified as T. versicolorbased on morphological features and sequence analysis using the NCBI BLAST database. Nutritionalanalysis indicated that T. versicolor contains carbohydrates (59.64%), protein (11.56%), fat (1.13%),moisture (7.1%), dietary fiber (8.6%), and ash (6.01%). The samples demonstrated notable levels oftotal phenolics (151.05 ± 0.002 mg GAE/100g), flavonoids (124.48 ± 0.03 mg QAE/100g), tannins(725 ± 0.03 TAE mg/100g), lycopene (0.46 ± 0.015 mg carotenoids/g), β-carotene (1.11 ± 0.02 mgcarotenoids/g), and terpenoids (2.78%). The antioxidant activity (IC50) was 198.4 μg/ml. Trametesversicolor also showed the presence of various minerals, including Cu (0.049 ± 0.0027 mg/g), Fe(0.105 ± 0.005 mg/g), Mn (0.035 ± 0.002 mg/g), and Zn (0.03 ± 0.001 mg/g). The mineralconstituents and rich nutritional and phytochemical profiles highlight its potential as a functional andnutraceutical food. This study supports its potential long-term utilization in healthcare and foodindustries, while establishing a basis for additional studies into its therapeutic implications.

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Abstract:

Pleurotus ostreatus, a mushroom of significant commercial importance, is extensively cultivated worldwide. The demand for enhanced mushroom strains with increased yield is on the rise. This study aimed to enhance mushroom strains using the dual-culture hybridization method. Out of thirty-four combinations of single-spore cultures, ten successfully formed hybrids, as confirmed by the presence of clamp connections. Both hybrids and parent strains were assessed for their performance in spawn running, pinhead formation, yield, and biological efficiency. Compared to the parent strains, the time required for the spawn run and the initiation of pinhead formation in hybrids were reduced. The hybrid strain KAL5×A9.1 displayed the highest performance, achieving a biological efficiency (BE) of 79.61%, surpassing other hybrids and the parent strains. The color of the hybrids fell between those of the parents. Additionally, the hybrids exhibited greater stipe length and pileus diameter compared to the parental strains.

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Abstract:

Ganoderma has long been regarded as one of the most important medicinal mushrooms, particularly in China, Japan, and the Korean Peninsula for millennia to enhance longevity and health. Ganoderma and its allied products are a multi-billion-dollar worth industry worldwide. Ganoderma, which is considered an important non-timber forest product (NTFP) in Nepal, has entered the industrial market only a decade ago. Besides the global market, the domestic market has grown dramatically in recent years. Ganoderma is collected in large quantities (about 4-10 tons per year) from Nepalese forests to meet its rising demand and is mostly sold to China in crude form. Since the authority has poor knowledge of the Ganoderma market it is transported with minimal royalty of US$ 0.043/kg under the heading entitled “sadharan chyau”. Almost all of Nepal's Ganoderma comes from natural stands, with the bulk coming from the Karnali (46%) and Far West (50%) provinces. With the great potential for the Ganoderma business, most of the other areas of the country have yet to be fully explored. By establishing processing units and offering locally priced micro-enterprise technologies, there are several opportunities for value addition. However, it looks that sustaining a steady supply of this highly sought mushroom will be tough. Concerns have been expressed about overexploitation of wild Ganoderma obtained in an unsustainable way or at an early stage. For the long-term management of Ganoderma and its habitat protection, a solid local resource monitoring system and scientific intervention for artificial cultivation are required. This study attempts to summarize the trade dynamics and development of the Ganoderma industry in Nepal with reference to the global Ganoderma industry.

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