Development of improved strains of Pleurotus ostreatus with a shorter harvesting period and a higher yield through hybridization
Abstract:
Pleurotus ostreatus, a mushroom of significant commercial importance, is extensively cultivated worldwide. The demand for enhanced mushroom strains with increased yield is on the rise. This study aimed to enhance mushroom strains using the dual-culture hybridization method. Out of thirty-four combinations of single-spore cultures, ten successfully formed hybrids, as confirmed by the presence of clamp connections. Both hybrids and parent strains were assessed for their performance in spawn running, pinhead formation, yield, and biological efficiency. Compared to the parent strains, the time required for the spawn run and the initiation of pinhead formation in hybrids were reduced. The hybrid strain KAL5×A9.1 displayed the highest performance, achieving a biological efficiency (BE) of 79.61%, surpassing other hybrids and the parent strains. The color of the hybrids fell between those of the parents. Additionally, the hybrids exhibited greater stipe length and pileus diameter compared to the parental strains.